FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634747
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG1001.559924E-524.1546061
TATACCG1602.1991582E-923.7176675
AGTGCGC900.005733161719.165798
TAGACCG1351.5760818E-417.8880715
CATAGAC1200.001475464217.2505723
GTCTTAG1503.5169072E-416.103071
CTTAGAC1756.347632E-515.7719523
TAGGTAG1554.5217143E-415.5799345
CTAGTAC1808.073998E-515.3338393
TATTAGG3503.765308E-1014.7873692
CTATAGG1657.254693E-414.6391551
GTATAAG3101.8040737E-814.4705011
ATACCGT2403.9978513E-614.3743456
GTCTATA1951.5939356E-414.1565461
GTATACG2202.8017113E-514.1163291
GTACTAG2454.937747E-614.0843181
GTATTAG3202.7392161E-814.0182971
GTCCTGA1500.006516352313.8026311
GAGAGTC1550.00810770313.3542297
GTATATA8850.013.2567651
Produced by FastQC (version 0.11.2)