FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261012
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2650.023.4354551
TATTAGG2450.022.5285632
CTAGACT1551.0062267E-620.0304344
GTACTAG1801.9821164E-719.1678891
GTATAGA5250.018.4011731
TAGGTAG2255.5497367E-918.3979175
TATAGGA2458.039933E-1018.3044572
GTATAGG4150.018.2903231
CCCTAAG1351.5770206E-417.8900321
TATAGAG5600.017.8644472
ACTGTGC3700.017.7146038
CTATACT2156.607115E-817.6495574
CATATAC3700.016.7822573
ATACTAT3501.8189894E-1216.7552476
TCTAAGC3106.002665E-1116.6920283
ATATTAT4000.016.3885461
CCATATA3601.8189894E-1216.2901842
CTATAGA2552.6000635E-816.2363321
TCCTAAC2351.8214268E-716.1474672
TTAGGTA3006.82121E-1016.0985364
Produced by FastQC (version 0.11.2)