FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948786
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT6950.018.36676269
TATAAGG4900.018.3055112
TATACTG6800.018.2631575
TAGACTG3205.456968E-1217.2485375
TAGGACT2753.7325663E-916.3081264
TACACAG3850.016.1285025
CTAAGCA4100.015.9868594
CTAGGAC2603.306195E-815.9221353
TATTAGG3703.6379788E-1215.85082152
TAGGATA3603.45608E-1115.3324284
CCTAATA2959.44965E-915.2028822
TCTAAGC4201.8189894E-1214.7848413
ATATACT6200.014.46688654
TAAGATA3403.9963197E-914.2050424
GTACTAG5350.014.1875331
GTATATA5600.014.170261
GTATAAA4202.7284841E-1113.9648931
TAGTACT4202.7284841E-1113.963464
GTATTAA3253.378409E-813.8006011
ATAGGAC3253.3829565E-813.7991843
Produced by FastQC (version 0.11.2)