FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542836
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC9850.020.3144843
GTATTAG9400.020.1875231
GTATAGA11650.018.6579021
TAATACT13800.016.249814
TACACCG7700.016.1293645
CCTAATA9000.016.0998122
TATATAG8750.015.7709345
TATACTG13650.015.4170985
TATTATA12200.015.2703132
CTAGGAC4550.015.1646583
TAGACAG8400.014.785255
TAGACTG4700.014.680395
CTATACT8950.014.6478734
TATACAG10450.014.5258615
TAGGTAG5500.014.426825
CCTAGCG3901.2732926E-1014.15618630
TCTATAC9750.014.1536813
TACACAG7100.014.0911075
CATATAC10050.014.0744623
ACTATGC11350.013.9816668
Produced by FastQC (version 0.11.2)