FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332908
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT451.0001236E-438.33275266
TACTAGG400.002593891234.4994742
GTCCCGT450.00461836830.666211
CCGCACC605.414517E-428.74956165
TCCGCAC800.002895410621.56217264
ACTCACT800.002895410621.5621728
CTAATAC2151.382432E-1020.8601473
ATCACAC1056.0112885E-419.71398529
CCTATAC900.005726547419.1663763
GTGTATG1301.17796735E-418.5766411
CGATTGT950.0078237618.15761814
CCTGATC950.0078237618.15761838
GTATATA5350.017.410951
CTATACA1250.001936910116.5597482
CATACAC1250.001936910116.5597483
GTAGTGG1250.001936910116.5597489
TATTAGG2109.924188E-716.4283222
CTGGTCA1300.002516922315.9228359
TATACCT1756.33574E-515.771194
TCTTAGA1350.003236913115.33312
Produced by FastQC (version 0.11.2)