FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094475
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44650.14428941904523385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10850.016.5348681
GCTTAGG13400.013.64467268
CGGTCGG2557.5106964E-613.5283513
CTAGACA8400.013.1414014
CTTAGGT13500.013.03252269
CGCGCGT4308.238203E-912.0335510
CCGCGCG3205.906635E-611.8580619
GTATATA22050.011.7349111
GTATAAT16550.011.4654691
TATTAGG11500.011.3989222
GTGTAGG5502.0918378E-1011.2911181
GTATAGA13250.011.1964341
CGAAGGC41500.011.13906669
GTCCTAA6900.011.0002031
TATAATA29850.010.9788612
GTATATG14200.010.9333011
CTTACAC6008.185452E-1110.9239663
AGGCTTA25100.010.85788969
TCTAGAC7650.010.8225063
GTATTAT19200.010.7814491
Produced by FastQC (version 0.11.2)