FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805708
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG5350.018.056321
GTGTAAG2802.5465852E-1017.2502371
ATAGGAC3850.017.0252633
CTAGGAC1907.0262286E-616.3414233
GTATTAG4750.015.9791661
TATAGGA4150.015.7945222
GTATTAT9050.015.2488281
TAGGACT2756.582559E-815.0539184
CCTACAC3252.1136657E-914.8609193
GTCCTAC2605.7239595E-714.5963531
TACACCG3851.03682396E-1014.3370645
TATAAGG6050.014.2556032
TAATACT7850.014.0631074
TAGACTG3751.0677468E-913.7994235
ATAATAC8050.013.7137133
TAATACC6550.013.6940854
TAGGACA4557.2759576E-1213.6477824
TCCTACA4355.0931703E-1113.4821962
TCGCGAC1550.0081165813.35391113
TATTATA6300.013.1423082
Produced by FastQC (version 0.11.2)