FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998655
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7700.021.9523075
TCCGCAC5350.020.63444164
GTATTAG12900.020.0574321
CGCACCT5450.019.62283366
CTAATAC14150.016.8216063
TATTAGG13650.015.92145252
CCTAATA14150.015.846442
TAATACT21950.015.7159384
GTATAGG9800.015.4892491
CCGCACC6950.015.3876965
TAGTCAG7500.015.1781989
ACTATGC13850.014.9440768
CGCGCAT3705.4569682E-1114.91913840
GACGCGC3756.730261E-1114.72046238
GGTAGTC9150.014.7031737
GTATAAG13500.014.3106071
TATACAG18100.014.2941225
GTATAGA13900.013.898791
TTAGGTA13500.013.79859354
GTATATA24700.013.5481041
Produced by FastQC (version 0.11.2)