FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930518
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA6950.020.3560411
GTATTAG4450.018.609961
TAGGTAG3103.6379788E-1217.8058435
TAGGACA3350.017.5073174
TAATACT7550.017.3640964
GTATTAA5050.016.3988781
TATAGAG6750.015.8443132
CTAATAC5100.015.5586943
TACACTG4000.015.5244695
CTACACT3151.3587851E-915.3332074
TATACCG4750.015.25211055
TACACCG4800.014.374515
TATACTG7200.014.3745095
CCTAATA4600.014.2498832
GTATTAT7750.014.2476261
GTATATG6850.014.104631
ATAGGAC3708.731149E-1013.986373
TACACCA6200.013.9108145
GTATATA10450.013.8684291
TTAGGTA4505.456968E-1213.7998864
Produced by FastQC (version 0.11.2)