FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462193
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6120.13241221740701395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGC553.2549346E-431.3621648
ACTAGCG850.00411763720.2931633
AGAGGGT1056.0174236E-419.7133586
TACTCCC950.00782967218.157045
GTATAGA2156.562004E-817.6522431
TCAGAAC1653.8351292E-516.7264863
GTTCTGG1452.7167294E-416.656191
TCCCGCT1250.00193745416.56101234
CTAGTAC1250.001938856416.5592213
GTGTATA1704.947988E-516.2362861
GTATATG3502.1827873E-1115.7723941
CCAATAC1554.5184465E-415.5799133
ACTGTGT1350.003240140915.3326128
TAAGGCT1350.003240140915.3326124
CTAATAC1808.071937E-515.3326123
AAGTCAC1350.003240140915.33261210
GTCTATA1605.746158E-415.0946731
GACAGAG1400.004127160614.7850197
TACAGTC1400.004127160614.7850197
TATAAGA3554.656613E-1014.5767792
Produced by FastQC (version 0.11.2)