FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059596
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA10300.017.7515871
CTAATAC9750.016.6291663
GTATATG12400.016.1363321
GTATTAG8800.015.2890491
TATACTG14800.015.1503185
GTCCTAA5850.014.742891
CCTAATA10150.014.2744862
GTATATA16400.014.0938451
TAGGTAG5150.014.0663755
TCTATAC12250.013.5170633
TATATAC15900.013.4515333
GTGTAGG6450.013.3714591
CCTAGCG4406.002665E-1113.33174730
GTACTAG8900.013.179151
TACACCG6850.013.0934155
CTATACA10900.012.9756014
TTATACT13150.012.8540634
GTATAGG9950.012.8285381
TATACAG12700.012.7662775
TACACAG8950.012.7192365
Produced by FastQC (version 0.11.2)