FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410065
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4730.11534756684915806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC700.001332315224.64307665
GCTACAT700.001334210724.6370668
TCCGCAC850.004115523320.29429664
AGTCTAC900.00573604419.1621610
CAGGACT900.00573604419.162164
CTTACTC1108.2373974E-418.8137593
GTATATA6050.018.2436451
AACACCG1150.001110318317.9957685
TAGAACC1200.001476909617.2459454
AGAGTGT1452.7219357E-416.6512586
TATAAGC1452.7219357E-416.6512582
GGACAGT1250.001940792116.5561076
ACTATGC1554.5233621E-415.57698258
TACTGTG1350.003243368115.3297287
TACTGTA1605.7571294E-415.0902022
ATACTGC2554.5827437E-714.8788546
GTCTAAT1901.2810041E-414.52290251
TAGATAG1450.005215443714.2725075
TAATACT4751.8189894E-1213.7967574
TACACAG1500.00652955513.7967575
Produced by FastQC (version 0.11.2)