FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212249
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93320.29051297081888733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGT20350.017.80070569
TAGGTGT15150.017.07894169
GTATTAA12000.015.2370251
TTAGGTG19800.015.15885569
GCTTAGG25250.015.029469569
TATACAG12650.014.9983655
GGCTTAG36850.014.792171569
AGGCTTA47550.014.22059969
TTATACT15550.013.75412754
GAAGGCT80850.013.61203469
GTATAAA16250.013.587271
TATACCG7400.013.5187965
GTATATA18200.013.4583721
TAATACT18200.013.4573254
CTAATAC14200.013.3612213
TACTATG12950.013.3190137
GTATTAG10700.013.2192141
ACTATGC13200.012.8054238
CCTAATA12200.012.7240232
TATTAGG10800.012.4569762
Produced by FastQC (version 0.11.2)