FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5213088
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21250.018.185421
TAGGTAG10900.017.0905675
TATTAGG19750.014.84750652
TAATACT34800.014.4734194
GTATAGA23700.014.121721
CTAATAC21750.013.7994473
TATACAG24600.013.6027235
GTATATA35550.013.296741
CTAGGAC8100.013.2031753
GTGTAAG11300.012.824341
GTGTAGG8100.012.7791041
ACTATGC22350.012.6566058
GTATAAG19950.012.6253711
GTATATG25600.012.5345051
GTATAGG18200.012.5122881
CCTAATA20350.012.3754252
TCCTAAG13800.012.249512
TATAATA42400.012.1233362
TAGATAG19550.011.9991745
CGCACCT10950.011.97223866
Produced by FastQC (version 0.11.2)