FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219555
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15000.014.2599241
GTATAAT21300.013.2816181
TAGACCG10100.012.6371985
CTAATAC17550.012.3836683
ACTATGC16350.012.2368738
TAATACT25150.012.2076694
GTATAGA16200.012.13882351
ATATAGA27350.012.1096251
CTATACT15400.012.0963354
ACTGCGC5753.6379788E-1211.9983728
GCGCACT5955.456968E-1211.59506511
CGATATG46600.011.474853568
GTATAAG15350.011.4624791
TATAATA28400.011.4182782
GATATGC45900.011.27404969
GCAGTGT17850.011.20962569
TATACTG21050.011.1436445
GTATTAT23050.011.0758631
CCTAATA14450.010.981992
GTCCTAG5057.530616E-910.9306351
Produced by FastQC (version 0.11.2)