FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875916
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA450.004615421430.6757334
GCTGTGC1151.6967315E-624.0002388
GGCTAGG1154.6006753E-521.0002081
CCGAGCA900.00573302119.1668579
TCGGGTG1108.237198E-418.81729525
CCGACTA1501.6938555E-518.39913214
GACCGTC2105.0322342E-818.0716087
ACCGCAC1200.001476864617.24918753
TCTATAC4250.017.0472283
CTATACT3257.2759576E-1216.9847834
GATCGGG1452.7224675E-416.65438723
CACTCGG1250.001940726916.5592249
CGGGTGG1503.525198E-416.0992426
TAAGACT2804.7239155E-916.0180174
CGACTAG1300.00252186215.92232615
GTATTAG3703.6379788E-1215.8515081
CATACAC2855.971742E-915.7369983
TCCAGGC1605.7556917E-415.0938993
CGACGTA1605.7581865E-415.09303845
CGTAGAC1400.00412952314.7858613
Produced by FastQC (version 0.11.2)