FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141058
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG1954.6059904E-717.6892499
GTATTAG8700.017.0515611
TATACTG12250.016.8958265
CTAGGAC3058.62201E-1015.8340653
CCTAGGG1554.5307341E-415.5786793
CCTACAC3603.6379788E-1115.3313983
GTGTAAG5200.015.2594711
ACTATGC8200.014.72306258
GTATATG12250.014.3631291
ACAGTCC3657.1486284E-1014.1756318
TAGTCAG4403.6379788E-1214.1111969
CCTAGGC1500.006536721313.7982573
GTATAGG6600.013.590781
TAGGTAG4355.0931703E-1113.4810565
GTATAGA11600.013.3834931
TATTAGG7800.013.2678652
CTAATAC8850.012.86278253
GGTAGTC4902.7284841E-1112.6712787
CCTAGCG3002.8581107E-612.65342630
GTAGGAC3002.8708528E-612.6484023
Produced by FastQC (version 0.11.2)