FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005204
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33140.11027537564837528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6750.020.952575
GTATTAG12800.019.9484441
TATTAGG13000.015.6565042
TATACCG9050.015.2464465
GTATAGA13700.014.6081331
CTAATAC13550.014.5110883
TAGTCAG7700.013.8876319
TATACGG10200.013.865675
CCTAATA14550.013.7515322
TCTATAC14650.013.6569833
TATACAG19150.013.5098235
GTATAGG11400.013.3178811
ACTATGC12350.013.1276848
GTATTAT24300.013.063781
GTCCTAA5850.012.9763971
GGTAGTC8000.012.9356577
TAATACT22250.012.8678294
GTATATA29700.012.7797831
CTAGTAC7100.012.6322143
TACACTG10050.012.3564475
Produced by FastQC (version 0.11.2)