FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103465
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32480.10465721379168122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC14300.016.8874233
TAGGTAG7150.016.405195
CGCACCT7000.015.77129466
GTATTAG13300.015.5652791
TATTAGG13750.015.5559792
TCCGCAC7000.015.27868764
CCTAATA14700.014.3159722
ACTATGC15950.014.0589878
GTATAGA14350.013.9454781
GTATATA32500.013.9073781
GTATAGG9950.013.5238041
GGTAGTC8450.013.4728227
CCGCACC8200.013.46329865
TAGTCAG7950.013.4522819
TACACCG7500.013.3396695
CTATGCA13750.013.2976399
TTAGGTA12350.013.1290184
TAATACT22050.012.9858654
TATACAG21100.012.7532395
TATACTG20850.012.4097655
Produced by FastQC (version 0.11.2)