FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514692
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6170.11987751898222627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGC350.001347768639.4247410
CTGAGCG703.8494793E-529.5685569
GGGTATA1551.8189894E-1228.9354861
CGTCACG500.007723685427.60536634
GAGTCCG750.001992661522.9977659
CGCACCT1154.5962926E-521.066
TCCGCAC1056.016791E-419.71428764
CCGCACC1056.016791E-419.71428765
CTTACAC1108.233666E-418.8163533
GTCGAGA950.00782434118.1596659
CTGAGCC950.00783311418.156139
ACTGAGC1150.001109814617.9982518
TGAGCGT1150.001109814617.99825110
TAGACTT2156.578193E-817.6494465
AGGTAGT1855.440186E-616.7821546
TAGGTAG1855.440186E-616.7821545
TCTTAGG1452.7207678E-416.6535532
TTAGAAC1958.971347E-615.921533
CTAATAC2907.501512E-915.4640143
TGGCTGC1350.00324189115.33184314
Produced by FastQC (version 0.11.2)