FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470397
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28450.11516367612169219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG1804.2035954E-617.2485075
TATACCG7300.016.0671025
CAGTGCG2454.9659047E-614.0804149
GTATTAG9600.014.0158311
GACTAGG3708.749339E-1013.9852758
TATACGG7600.013.6172425
CCTAGCG3052.3315988E-713.57479430
GTATAGA10350.013.33352851
TATTAGG11250.013.1857932
TCTAGAC5750.012.598913
GTCTAAG6700.012.3583911
CTATGCG3154.9525424E-612.04657659
CTAGACC5552.0008883E-1111.8097894
GTATATG13800.011.7501731
TAGGCAG4702.4429028E-911.74366611
GTATATA24250.011.666151
CATATAC14600.011.5777653
ACTATGC9250.011.5611628
GTATAAT17400.011.5001691
ATTAGGT11450.011.4487913
Produced by FastQC (version 0.11.2)