FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926226
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21950.11395339903002037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC7550.015.5355543
GTATTAG5300.014.9712471
CCTAGGG1657.2777714E-414.6355433
GTATAGA8950.013.87667751
GTATATA15750.013.58054351
CTAGGGT1850.001726852713.0533234
ACTGTGC3457.395647E-812.9992718
GTATAGC5350.012.8968081
CCTAATA8050.012.8564222
TCCTAGG2153.6577397E-412.836492
GTACTAG4357.203198E-1012.6892731
ATACGGT5750.012.5992946
CTATGCA6600.012.5447519
TAGACTG4256.9121597E-912.1757885
CCTACAC2852.356674E-512.1045853
TACACAG5451.4551915E-1112.0268495
ACTAGAC3451.0408949E-611.9993283
CTGTGCA4359.760697E-911.89588459
TCCTAAG3501.2383116E-611.82790852
GTATAAA12850.011.8128741
Produced by FastQC (version 0.11.2)