FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468974
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32140.1301755304025073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5800.017.8443095
CTAATAC11300.014.9597423
TCCGCAC4450.014.73050764
CGCACCT4750.014.52618966
GTATTAG11700.013.8608181
TAATACT17850.013.7222914
CCGCACC5100.013.52956765
GGTAGTC5750.013.1998837
GTATAGA12200.013.0099261
CCGTGCA2952.3752254E-612.8642939
TATTAGG12150.012.7774072
TATACAG15200.012.7101565
GTATATA27850.012.6372281
CTTACAC5750.012.5996333
TAGTCAG6300.012.5951279
TATACCG6850.012.5908755
TGGACAG7900.012.2274935
TATACGG8500.012.1761175
CTATGCA8650.011.9652139
ACCGGTC2902.815491E-511.8964468
Produced by FastQC (version 0.11.2)