FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316709
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.11114303666772969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTG400.00259519634.49542221
GCAAGGT750.00199139122.99694611
AGGACAT1004.324523E-420.7005215
GTCTAGA850.004113100520.2946281
CCGAAGA950.00782824218.15548313
TGTGTAG950.00782824218.15548310
TAGACCA1402.0761814E-417.2504355
ATAGTGC1200.001474893217.2477118
TTAGACT1452.7140847E-416.6555924
TTACACA1503.5143836E-416.1004054
GTATTAG1958.9391615E-615.9234771
TAGGAGT1300.0025158815.9234774
TATACTG2603.2727257E-815.9234775
GTACATA1756.3319916E-515.7718251
GTCAGTA1350.003235576215.3337192
TAGAACC1400.004121367414.7860874
AGTTTGG1750.001130099113.80252631
GTATTGG2052.4334186E-413.4637541
ATTAGAC1550.00809214713.3551753
AAGAGCT1550.00810060113.35306559
Produced by FastQC (version 0.11.2)