FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907893
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59190.15146269357937897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13300.014.008071
TATACAG18300.013.5727415
GCTTAGG16050.013.54172268
GTATAGA16950.013.4341561
CTTAGGT16850.013.30827869
GTATATA31250.012.5860941
GTCCAGG6100.011.8775171
CTGGGCG4451.3687895E-811.6283329
TATACGG11700.010.909435
TATACTG21450.010.7754075
ATTATAC25250.010.6566173
GAAGGCT44000.010.34974469
TATTAGG17700.010.3297242
CGAAGGC43850.010.30647368
GTATTAA19850.010.2548111
GTATAAT22550.010.25095751
GGCTTAG22050.010.16981767
TAGGTAG8150.010.1587385
CCTAGCC3753.4221408E-510.1192323
GAGTGTG10600.010.0888577
Produced by FastQC (version 0.11.2)