FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112210
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42260.13578775211184335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA13250.015.8837151
ACCCGGG2052.446548E-413.4622746
GGACCGT3204.1900785E-712.9364036
CCGGACG2406.379845E-512.93619512
GTATTAG12250.012.6740251
GTATATA27500.012.2950841
GCTCGGC2601.3479567E-411.94110411
CCTAGCG4207.637027E-811.50253630
TATACCG9300.011.4990255
ATACTCC28400.011.41859769
TACACCG5158.440111E-1011.3873845
TATACAG16450.011.3242685
CGATACT34900.011.26892969
GTATAAA22700.010.9432111
TAATACT20300.010.7059894
TATTATA33400.010.5352052
GATACTC30900.010.49476268
ACTATGC11700.010.3194728
CACCGCG3055.9117825E-410.17946523
CTATACA13250.010.1538564
Produced by FastQC (version 0.11.2)