FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341759
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48260.1444149622997948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGT13500.022.23284169
TTACGTG20050.020.64792369
CCGACGC6850.017.62734669
CCTCGCG2652.2882887E-916.9221299
CTTACGT27200.015.22025168
TATACGG7400.013.5193625
CCTTACG33350.012.93074869
GACTCCT80750.012.85977769
GTATTAG11000.012.5461151
ACTCCTT75200.012.34081869
ACAGTCC5105.638867E-1112.1747458
CTCCTTA53000.012.17237369
GCGGTCC2306.4623414E-411.9982999
CCTATAC9250.011.9343363
TCCTTAC46200.011.87311469
TTCTAGG9900.011.8480452
TAGACAG9650.011.7971375
CGACTCC92100.011.76195569
TATACAG13050.011.3670545
GTACTAG7950.011.2836131
Produced by FastQC (version 0.11.2)