FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837975
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38620.13608294646711125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG6000.016.099019
CGCACCT4950.015.33266166
TATACTG19000.015.2516935
TGTACAC9400.013.9464413
CAGTGCG3506.013579E-913.799159
GTATTAG15650.013.4478781
TCTAGAC7700.013.4412063
TCCGCAC5400.013.41678664
CTATACT13200.013.328494
TACACCG5700.013.314975
CCGCACC5500.013.17214965
TAGGTAG7650.013.0776275
TATACCG8600.012.8364195
TATTAGG15150.012.7523642
TCCTATA10850.012.7187842
GGTAGTC7350.012.6726897
CTAATAC13050.012.424963
GTATATA32050.012.3796431
GTATAGA12900.012.302871
CCTATAC8700.012.292783
Produced by FastQC (version 0.11.2)