FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725807
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24940.14451210361297642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT3054.7293724E-1116.9657024
ACTGTGC4100.015.9863838
TACACGG2202.8146293E-514.112385
TATACGG7550.013.707395
ATACGGT6800.013.69730956
TATACCG5800.013.6798175
CTTAGAC6100.013.5725623
TAGACAG6150.013.4622165
TACGGTG6450.013.3709037
TAGGTAG4457.2759576E-1113.1786035
CGCACCT4002.8030627E-912.93672366
TCTATAC8900.012.79099753
CTATGCA7050.012.7222729
TCCTAGG1900.002110244512.7097642
CCTATAC5700.012.7093943
TATACTG13650.012.6362385
GTATAGA8750.012.6182141
AGGACAG4953.274181E-1112.5443385
CTAATAC8550.012.507663
TAGACTA3601.2891178E-712.4572255
Produced by FastQC (version 0.11.2)