FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729977
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56420.11928176394938071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG19350.014.2621995
TAGGTAG12050.013.7414225
TATTAGG22150.012.9269372
ACGGTGT16750.012.7689278
TACACCG9000.012.2654925
TATACTG33800.012.1452875
TCCTATA17550.011.99068452
GGTAGTC10050.011.6705247
GTATATA49600.011.6156751
TACGGTG17950.011.530937
CTAATAC22450.011.5246313
TAATACT37350.011.452844
ATACGGT19900.011.4411156
GTATTAG19750.011.3541831
CTATACT19650.011.0601264
TAGCCCG3451.363159E-510.9989475
GTATATG32400.010.9673541
TAGACAG14350.010.817785
TATACAG29800.010.7657475
CATATAC34600.010.6681673
Produced by FastQC (version 0.11.2)