FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367290
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4610.12551389909880475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAT500.00773100627.5979336
TAGGACA851.441424E-424.3511144
GGTCTAA953.0588623E-421.7908061
TGTAGAC1304.9099654E-621.2291773
TCTAAGC1258.7254186E-519.3185523
CTAAGCA1301.17892785E-418.5755314
TGATTCG950.00782795418.15653417
CATAGAC1250.001938196516.558763
GGACAAG1250.001938196516.558766
TATACTG2654.1478415E-815.621475
TCTGTAC1554.5162614E-415.5794773
TCAATCG1350.003236114515.33427135
ACTGTTC1605.74811E-415.0926198
ATAGACA1657.259016E-414.6352654
TTAATAC2403.9880106E-614.3739223
AGCTGCA1450.005203831514.27673652
GATCCTG1450.005208536514.27479311
CCCAAAC1450.005208536514.2747933
GTTTAAG1500.0065150513.8008441
GCCGACG1500.0065150513.80084468
Produced by FastQC (version 0.11.2)