FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211242
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT35430.16022669612823925No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG25460.11513891288244345No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCA23380.10573243453226738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACG7250.023.3178777
CCACGCA7050.022.9995849
GGGGGGC5900.022.2209438
AGGGGGG6200.021.7022047
CCCGATC3700.021.44604538
CCCACGC7750.020.923158
GGGGCTC6550.020.54161510
CGAGTGG4050.020.44453813
CCGAGTG4050.020.44407712
TAAGGGG6600.020.387385
GGGCTCA6600.020.38599611
CCTAGTC4100.020.19521345
GCTCATG6650.019.71437613
GTCCTAA6700.019.568581
TCCCTAG4400.018.81869543
CTAGTCG4250.018.67067346
CTCGTAA4650.018.549731
ATTCGCC20050.018.41155616
GATCCCT4600.018.00008241
GTCGTGA15650.017.85631231
Produced by FastQC (version 0.11.2)