FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138786
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT23270.10880003890057256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACG4200.024.6426567
CCACGCA4800.022.2810699
ATTCGCC13300.020.23291816
GCATATC5700.019.369171
CAGTGCG1451.2657241E-519.0343279
GAGCCGT1702.4344517E-618.2645576
GGACCGC950.00783696818.1577456
AATTCGC14500.018.08261115
CTCGACT6500.018.04642925
CTGGCCT3650.017.958764
GTCATAT5250.017.7435421
CGGGCGG5150.017.41733452
AGACCCC3257.2759576E-1216.98487523
GCGTCGC9600.016.89127733
GCAAGCA16200.016.82433124
CATTGCA15800.016.8131549
GGATACG9700.016.7163589
CGCAGTT6400.016.71080261
CCCACGC6200.016.693418
CCATGCG10150.016.6550379
Produced by FastQC (version 0.11.2)