FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169756
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA150.002353196768.991753
TAGACCG200.007351766351.7438135
ATCGTTT200.007351766351.74381345
TAGGTTG400.00259165134.4958765
GGGGAGC500.007721169427.59676
GGTCTGA750.001984050323.00402634
GAACTGA750.001987454222.997256
TACACTG800.002891379621.5599235
GGTTTCC900.00571867719.16437758
TATGCTA900.00571867719.1643775
TTATGCT1350.003230432315.33154
TATACTG1709.0675574E-414.2041855
ACTGTAC1550.008079587513.3532438
AAGTGAA1600.00996428412.9359537
ATTATAT3601.7095463E-611.4986263
ATACTGT2150.005290328511.2312156
TTATATA2900.0044183979.5161042
GTATATA2950.00508715779.3548141
CATCTCC4350.0016436577.930086669
ACATCTC4650.00316374637.41846868
Produced by FastQC (version 0.11.2)