FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610374
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG2550.035.1748434
CGCCGAG400.002593889134.50405532
GCAGCGT2700.033.2234081
CAGCGTC2900.030.9296022
AGCGTCA3150.028.4748733
CGTCAGA3150.028.4748735
GAGACAG3150.027.37968421
GATGTGT3500.025.62738410
ACACGGT750.001992493922.9989366
TGTGTAT3950.022.7078112
GTGTATA3850.022.4015613
CTAGGAC800.002898670321.5615023
ATGTGTA4200.021.35615511
GTCAGAT4300.020.8594996
AGACAGG2001.2769306E-920.69904122
GTCGCTG850.00411520120.29650335
CGGGAGC850.00411715420.29484226
ATAAGAG4950.020.21118517
AGATGTG4450.020.156379
TCAGATG4700.019.0842237
Produced by FastQC (version 0.11.2)