FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838008
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG25500.014.0704455
GTATTAG16600.013.5092511
GTATAGA16700.012.1888171
ACCGTGC5259.276846E-1111.828458
TACACTG12050.011.7384695
GTCCTAG5602.3646862E-1111.70554351
TCCGCAC5905.456968E-1211.69464364
GTATATA41600.011.6107611
TAATACT29350.011.5194734
ACTATGC16100.011.3570278
TGGGGGG7300.011.342355
TCTATAC18750.011.2240313
TATACGG12350.011.1739755
TATATAC47100.011.0605413
TATACCG11300.010.9910385
CATATAC26700.010.9831773
TAGGACC8100.010.6480394
AGGACCA11750.010.5701055
CCGGCCC4251.1058455E-610.55269513
TATTAGG19700.010.5077812
Produced by FastQC (version 0.11.2)