FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658811
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG5050.016.3961185
TGTACCG2452.9197145E-715.489875
ACCGTGT2454.9588507E-614.0816998
ACCGTGC2253.474755E-513.8000668
TACCGTG3253.3789547E-813.8000667
TGGGGGG2609.163101E-613.2692945
TAGAGCG2355.2287447E-513.212835
TACCGGG2102.9963374E-413.142927
GTACCGT3701.3082172E-813.0541166
GTCCTAA4601.2732926E-1012.7512151
CTAATAC9300.012.6133443
GTATTAG7450.012.5045471
CGCACCT3053.4491895E-612.44230766
CGTCCGT1950.002562141512.38542236
TAATACC7850.012.3061624
TCTAGGG2255.361189E-412.2670963
GTATATA19700.012.260051
AGACTGC3408.7168155E-712.1765296
ATACCGT6800.012.1765296
CGTGCCA2651.6075894E-411.71668410
Produced by FastQC (version 0.11.2)