FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871504
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1108.2430086E-418.8169449
ACCGCGC1653.84408E-516.7270668
GTATAGG5650.015.8754841
GTATATA17400.015.4649111
CTGATCG2252.0507832E-615.3323249
TCCGCAC3857.2759576E-1215.23276264
GTATTAG7650.014.8817711
GCGTGCA1400.00413100114.78632141
TCTATAC8250.014.6365733
CGCACCT3808.367351E-1114.5253666
GTCCTAA4550.014.4060311
CGCCGAG1450.00520954214.27873732
TAGACAG6300.014.2382995
GTATAGA8000.014.2306931
AGTCCGG2708.7746594E-714.05463110
TGGGGGG3304.128742E-813.5911045
ACCGTGC2557.501787E-613.5292438
CCGCACC4406.002665E-1113.32866965
GTCTTAG4153.4197E-1013.3006851
TCGGTCG2102.998449E-413.1419929
Produced by FastQC (version 0.11.2)