FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAATACCGGATCAGTGGCACCTATTGAAATGCTAAAAGCCGCAATTAATGCTTATAATGAAGTAAATAGACC11.2987012987012987No Hit
CTCTTATTGTAGACCAACCCCACCCTACACCCCCTCAAACCGAGGACACTTCAAAAGGAAAATCAACAACTTTGG11.2987012987012987No Hit
ATATATTAAGCATGGAGCAGAGTTCACACCTTATACTTAAACTTATCGTTTCTGCAAAGTGCGGTGTTTTTAGTA11.2987012987012987No Hit
AACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCGGTATGCGGAGTTGTAA11.2987012987012987No Hit
CCCCATTAGTTTTCGTCTTATACCTTGAACCTTAACGGAATGTCTGTCACTAAAACCTTGTCAAATTACTAGGCA11.2987012987012987No Hit
TTATCTGATTCTGTCATGCTTTGTGACTGTCCCGGTCTTGTCTTCCCAAACTTTGCATATAACAAGGGTGAGCTC11.2987012987012987No Hit
ATCAAACATGCACCGAAAACAACCATATCAATCAATGTCTTGCTTCATTCTTGATTTCTCATCTCTTTCTTTCCT11.2987012987012987No Hit
ACTGCAATCTTTCCTTATCCTTGGCATTAGTTAAGGCGACTATTTTACCTTGTGGTACTATATTATACGGATTAT11.2987012987012987No Hit
ATTTGAGAGTAAGAAGAAGAAGAACAATATCAAGTTGTACGTTCGTCGTGTCTTCATCACTGATGAAGCTGAAGA11.2987012987012987No Hit
ACACAAGCAAGCCCATCTCTATACACGATGTAAAGTGCCACGGGAAACTAAACGATATCTGCAATGAAGAGCTCT11.2987012987012987No Hit
GTTCATCCACCTAGTGCGCCAGCTTTCCATACAAAGAGTAGGGGTTTGTCTAACAAAAACGACGATAGCAGTAGT11.2987012987012987No Hit
ATATGGAACACCCTGTCTGGTGACGTATCCAAATTGCTTTGATGCGTTACCAGTCCTAGCTAAAGAGTAAAGAAC11.2987012987012987No Hit
AAGTAATGTAATGATATACACTTTTTTTCTCAATTCTTTACCTTCTTCTTTCGTACGGTGTACTGCGAGAAGTAA11.2987012987012987No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAGGGAATCCCGATAA11.2987012987012987No Hit
AGAAAACCACATGCATAAAAGTCGCTTGCCACATAACTCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGC11.2987012987012987No Hit
AATAATTGCTCTTGCAGTCTCAATGAGTCGGTATGTTCAAAGTGCTGCCCAACATTTACTAATATTGGACTGCGG11.2987012987012987No Hit
ATATAGGGAAATTGGTGGGGGCTCCCCAATCCAGAAATGGTCTGAGTATCAAGCCACTGAGGTCTGTAAAATATT11.2987012987012987No Hit
GTTACGTTGTGGAAGGACGCTTTAAAATTAAGTGGAGACAGTAAACCGTGCTTCGGGTCCATGGGAGGGAACCCA11.2987012987012987No Hit
ATTTTATAATTATTATATATTAGTTATATTATTAAATATTAACTAATGTGTGCTCTATATTTATTGAATAGTTTG11.2987012987012987No Hit
CTTTGTTGGCTTTAGGCCAATCAGGTTGTTCAAGGCGGCTGGGACTCTACCAGAACCTGCTGTATCAGTACCCAA11.2987012987012987No Hit
ATATTATGAACGCCAGCAAGGAAATAGAACATCCAACTTGGGCCACAATAATCTTCCATTTTATCATTCTAATGA11.2987012987012987No Hit
ACCACCACGAGCCAACACAATATCACGGTATTGTATCCCATTCTTGGCATTTAACGGATCCTTAGCGAATTTGGT11.2987012987012987No Hit
GTCGTAATCCTTTCTATTGAATTTCAAAGTATGCACTTGAAACAACGTGTAGACCATCAAGTTGATTTTCTTGGG11.2987012987012987No Hit
GAAAATGAATGACAAAGTATAAAACGAGAAAGAAATGATAATAGCATTATGCGGAGATATCGGTTCCATTTTGTG11.2987012987012987No Hit
TATAAGAAAAGCATATAAACACGCGCCCAGAGAACATATTCAGTATGCCCTAGCTTGGAAACACATCAGTAAAAT11.2987012987012987No Hit
AGTATATTGTAAGAAATTTTTTTTTCTAGGGAATATGCGTTTTGATGTTGTAGTATTTAAATGTTTTGTTTTATT11.2987012987012987No Hit
ATAAATAACCGTCGAACCCCACAAAAATTGGTAATCTTTCAACCCAGCTTACAACTATTTCGGAACAAGGCATCT11.2987012987012987No Hit
GATAAATACAAATAAATTTAAATTTTCAATAAATATAAAATAAATATGATCAATAAAAAATATAACAAGTTCTAT11.2987012987012987No Hit
GGTAACTGTCAGAGTGATGACGCTTGAACTTCATGGTGTGCTTATTGACAAACTTTGGAAGAGGTAAGGAGGCCA11.2987012987012987No Hit
GTCTAAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCCATTTTACATTTGGAGGGACGG11.2987012987012987No Hit
ATTAAAGTATGGCACCAAACCCTTGGTCCAATTTACAGGAGATGAATTAAGTCGGCTGCGTAAAATTCGCGGGTG11.2987012987012987No Hit
CTCTAAAGTGGCCTTCAAAGTTAAATCCTCTGAGTGACATATGTTAGCAACATTTTTCCAAGTGAACACTTCGCC11.2987012987012987No Hit
GTATGTACACATATACTATATACAGGAAAAGATAAGCAAGAGAGAGGATATCAACTACGAGAGCGATCGATTATA11.2987012987012987No Hit
GGTCACGACAGACGACACCGATTGTCAGCGCTTCAACTACAACGCCAAGATGAATGGTGGAATCAAAACTGTCTC11.2987012987012987No Hit
CCTCGTAACTGTTTTCTGCCCATGAGTAAAATTTCAATAGCGCTATGGAAGGGGGGAGAAAGATTTAACCAGATA11.2987012987012987No Hit
GGATAAGCGTAAGAGTGCATTTGGAAACATTGGACATCGTCTTGCCTCTGCATCTTCCAGTCTCACTCATAATGA11.2987012987012987No Hit
AACTATGTTTGTGTAGTTATTGTTGGTTATACTGCGTATTCGATTCTTTACTGGAAATACAAGGGTAAGAAGGAA11.2987012987012987No Hit
ATTTGAAATAATTATGCGGATTGGGTCATCAACTCAACAAGCTCGTTCACGGGATCTTCATCGTGCCTTGCGGTT11.2987012987012987No Hit
ATTCATGTACATCTCTACATAGTATGATCGACTTCGGGATTTTTTATTCCTTACTACGTGGTTTTTACGATGGCA11.2987012987012987No Hit
CCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC11.2987012987012987No Hit
TTCTTGAATTGCCATTACGCTTCCATGCGGCATATTCTCGTGTAGTGTGACAAAAGGTGCGGGTGGCAAATAAAG11.2987012987012987No Hit
CCTCAAGAGAGTATGATGGAGATGAGACCTGCCACTGGAAATACCGCAGATATTCCGCCTCAGAATGATAACGGC11.2987012987012987No Hit
GTGTACGCGTAGTCGACACCCATACCGTACGCGACATAATGCTCCATCGCGATCGCCATCACGGCGTCGTACGTC11.2987012987012987No Hit
GAATTCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCATGACGGAGTTTCACAAGATTACCAAGACC11.2987012987012987No Hit
AACAATGCCAAAAATCATGTAGCTGCCCAACGGGGTGTAACAGCGACGACAAATGCCCCTGCGGTAACAAGTCTG11.2987012987012987No Hit
TAACAACCTATCCCAAGATGACGATGATATTGCAATTTACGCTGCAAATCCATTCAAGGATACAGAATTTGTTGA11.2987012987012987No Hit
GACAATAGAAGTTTCAAAAGAGTCACTTAATCCGACAGCCTGGAAAACAATGGTACCGTAGTAGAAGAAATAGTT11.2987012987012987No Hit
CTCGAACTGCTCGTGCGTATGCGTATGCTCATGCCGTTCGCGAACGTGCAGCCAGATGCCGAGCGCCATCAGCAC11.2987012987012987No Hit
AAATTACTGGCAGCGTTTAAAGCAACTGCAAAGGAGGATTTAAATCGGCATCGTAGAGATGAAATCAATTTTATG11.2987012987012987No Hit
CTAATCAAAAGAGGAACCAAAATCTTAGGTGAAACTAGGTTAGCGGAAGGCCTCTTTTTATCTATCTTTTCATAG11.2987012987012987No Hit
GTATTTATAAGAATCTCTTAACTCTTCATCGTTTGGTAAAAACCTTCTTGCGTTGCTTGGAATAGCCCTGTCTCT11.2987012987012987No Hit
GTCTTTACCTTCTAGTTTTTTGAACACATCTATGACGTTCAAAAATCTTAGCCAGATACTCGCTGATGACGGTGA11.2987012987012987No Hit
CTCTAGAAAATTGGTAATCTTCACGAATAATTTCTCTTAATGACAGCAGAATTCTTTCCGCGTAGTGAGGAGTCA11.2987012987012987No Hit
ATCTCATCATCTACCAATATCTCTCCGGCTAATGAAACACAACTAGAAATACCTGATACTCAAGAATTGCATCAT11.2987012987012987No Hit
GACAATAGAAGTTTCAAAAGAGTCACTTAAACCGCCAGCCTGGAAAACAATGGTACCGGAGTAGAAGCAATAGTT11.2987012987012987No Hit
CTCTTGTGCAATTCTGTGACGATAACCGTCCTAGAAGACAGCAGTCCGTCATAGAGTGGTGTTGTCTGCATAGCA11.2987012987012987No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTA11.2987012987012987No Hit
GTCAATACATAGTAGTGAGTCGCCTATAGCCATTCTGTAATGCCATTTGTTAAGACGTGTCCTTAAACCCTTCCG11.2987012987012987No Hit
GCAGCGTCAGATGTGTATAAGAGACAGTTCCATTACCAAGTGGTGAGCTCTTGCTATTAGTGATAGGTCATTAGT11.2987012987012987No Hit
GTATTATCAATGTGGTAAAGGTAGAGATTTGGGTTTCGGTACAATTCTAAATTTCACTACTAAGATTGGTGCTGG11.2987012987012987No Hit
AACTATAGTCTCTGCCGTTCCCATAATATTGATTTTTGGAGCAGCAGTTTTTACAAAAGAAAAGAAAAAAAATAC11.2987012987012987No Hit
CATATACATATACTTATATTTATCTATGATACATACCGCACTTTAAATAGTAACGACATACATGTGGTAATGAGA11.2987012987012987No Hit
CTTATTGCTATATGCTGTTCGACGCGATGAGATGATTACATGTTTTTTATTAAATTTCTCAAGCAGACTGCGGGT11.2987012987012987No Hit
CCACAGTTCTGTTCTCAATCATTTCCATTTGGATTATGTTCTCCTTCAACAACTTCAAAATAACAGTTGGTTTCA11.2987012987012987No Hit
CTACTATTGATGGGATTACCAAATTCATCCACTTCTTTATCTTCTCCAAAGTATGAAGAAGAGGAAATTGATGTA11.2987012987012987No Hit
CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTTTAACCTACTGATGAATGTTACCGCA11.2987012987012987No Hit
ATCCAGGACAGCCAGGACCTTTTCTTGCCAAAATTCTTGCTTTCCCATCTCAGAGAAAAAACGAGGAATTTTTCT11.2987012987012987No Hit
GCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTAC11.2987012987012987No Hit
ATTCTACATTGCCGCTGACAAACTATTGCCTATTGAAAGATTTTTACCTAAGCCTAAGGTGGTAGGCCCACCAAA11.2987012987012987No Hit
GTACGGTGTTACAAGATCTAACAGGAGCAAGAAAGGCTCAAATTCTGTCTATTGAAGACGAATCAAGCGTGTCAA11.2987012987012987No Hit
AAGATGTACTACGATCATATAGTGTAATGCGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACT11.2987012987012987No Hit
CGTAATGAATTAGCAAAAATCCCTAATAAAAGTTGGTTATGAAGTCGTACTAAACTATGGTGATGATTTGTATTT11.2987012987012987No Hit
TACCAATGCTAGTACTAATGCGACTACCAATGCCAGTACCAATGCGACTACCAACTCCAGCACTAATGGTACTAC11.2987012987012987No Hit
GCAATGGTCCGAGCAACAAAGCGCAGGCACAGGATATTGGAAACGCCGTTCTACAGGATCTGCAAGACCACCCCC11.2987012987012987No Hit
ATGTTTAGAATGAAAAACTAATGACTTAACGGGAATATGTCTTACATCTGTCTCTTATACACATCTCCGAGCCCA11.2987012987012987No Hit
AATATATAAATCAGCCACTGTTCCAGCACCTACACTTTGAACACTGGCAGAAGCAGCCCCACAGAGCATTCTCAA11.2987012987012987No Hit
GCGCATTAGTATTTGAGTTGTTATTGATAGATGGCTGGTTGATGTTGCTGTTGGACGTGCTGGAATTATACGGGA11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA50.069.014
GTAGAGA50.069.053
ACTGCAA50.069.025
TGGCAGC50.069.08
GGCATCG50.069.047
TAAATCG50.069.041
GAGGATT50.069.034
AAATCGG50.069.042
AATTTTA50.069.067
GAAATCA50.069.061
AAATCAA50.069.062
AGGATTT50.069.035
TTACTGG50.069.04
AAATTAC50.069.01
AACTGCA50.069.024
TGCAAAG50.069.027
AATCAAT50.069.063
CAACTGC50.069.023
TCGGCAT50.069.045
GGATTTA50.069.036