FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734059
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2156.598384E-817.6509784
TCCGCAC3600.017.24932364
TAATACT16000.016.387334
CCGCACC3753.6379788E-1215.63938665
CGACGGG1808.089884E-515.33317349
CTAGGAC3001.178887E-814.9498433
TCTAGAC4400.014.8975713
CTAATAC9750.014.86138253
CGCACCT3959.094947E-1214.84751766
TAGGTAG5150.014.737715
TATTAGG9600.014.73422052
TATACTG15000.014.4898485
TACGGTG7150.014.4753737
CCTAATA10500.014.4569912
GTATTAG8950.014.262011
TATACGG8000.014.2311015
TGTACCG3202.757406E-814.0154795
ACGGTGT7400.013.98634058
ACTGTGT5250.013.7998558
TAGGACT3751.0677468E-913.7998554
Produced by FastQC (version 0.11.2)