FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412653
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG7000.013.7998725
GTATTAG18200.013.45881751
GACCAGA40400.013.15071869
AGACCAG50600.012.88609769
GTATAAG16600.012.8855671
CTAGGAC7600.012.7104083
TAGACAG11950.012.702815
TATAAGG18750.012.6958822
CGCACCT8200.012.62154866
CATATAC28850.012.5562093
GTATAGA17450.012.4556121
CTAATAC22250.012.4043793
TATACAG21750.012.3722975
TCTATAC18150.012.3552573
ACTATGC16250.012.3137318
ACCAGAG36800.012.18724869
ACCGTGC5401.2732926E-1112.1387758
TCCGCAC7750.012.0189764
TATACGG14450.011.9376045
CCGCACC9250.011.93475265
Produced by FastQC (version 0.11.2)