FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452269
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC200.007357280751.7480110
CGCGCGG306.3085696E-445.9982343
GCGTCAG1350.040.8873184
GCAGCGT1650.033.453261
CAGCGTC1750.031.5416452
CGTCAGA1800.028.7488945
AGCCGGC500.007731491727.59893659
CCGTGTA500.007731491727.5989369
AGCGTCA2050.026.9257933
GAGACAG2450.022.52974521
GATGTGT2500.022.07915110
CAGATGT2350.022.020438
CCGTCCG800.002897389221.561679
TCTATAC1851.1721568E-820.5127263
GTCAGAT2553.6379788E-1220.2933396
TCAGATG2553.6379788E-1220.2933397
GTATACG1206.3780135E-520.1242261
GTATAAG2800.019.71352815
ATGTGTA3350.019.5664111
AAGAGAC2951.8189894E-1218.71114319
Produced by FastQC (version 0.11.2)