FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670644
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA14000.015.2781071
TCCGCAC4650.014.09608264
GTATTAG11150.013.9233431
TAGACAG8800.013.7214325
GTATATA24950.013.550691
TCTATAC11300.012.8228593
TACACCG6200.012.7982385
CCTAATA13800.012.7498512
CTAGGAC4651.546141E-1012.6127573
GTATAGG9100.012.5106091
CCGCACC5257.2759576E-1212.48510165
TATACCG7600.012.2564365
CTAATAC13050.012.1607783
GCCGTCT5301.09139364E-1011.71706346
CTAGACA7950.011.7168454
GTGTAGG4451.3669705E-811.62885951
TATACTG15450.011.6115155
CGCACCT5652.7284841E-1111.601266
TGGGGGG3903.645855E-711.4998665
GTATACG11550.011.3503041
Produced by FastQC (version 0.11.2)