FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744094
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC6050.015.3964058
CGCACCT5100.014.88207466
CTAATAC12300.014.8655753
ACCGCGC2708.7742774E-714.0552928
GACCGCG2708.7742774E-714.0552927
TAGGGGG2708.7742774E-714.0552924
TACACCG3951.5643309E-1013.9744225
TCCGCAC5450.013.92609164
CCTAGGG2506.1154205E-613.79999352
ACTATGC11000.013.7997418
CCGCGCA2557.5067383E-613.5289129
CCGCACC5700.013.31529865
TAGACAG7300.013.2326285
GTAGGCC4204.129106E-1013.1426113
CTGCGCG1850.001726930513.0535719
CTGTGCG3457.3983756E-812.9995199
TAGGTAG4551.07320375E-1012.8898695
TAATACT16650.012.8466074
TACACAG7150.012.5452195
ACGCGGT5004.0017767E-1112.41999351
Produced by FastQC (version 0.11.2)