FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294915
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT7950.013.45260546
TGCCGTC8100.013.20348345
CTAATAC14850.012.3127383
GTATATA24150.012.14283851
ATGCCGT8650.011.96511844
GTATAGA12200.011.8770311
ACTATGC12750.011.6347468
GTATGCC8900.011.62954842
GTATTAG13100.011.5877691
ATACCGT12250.011.5465716
GTCCTAA7250.010.944811
GACCTCT40050.010.93972669
CTATGCA9800.010.9127529
TGGGGGG5703.9108272E-1010.894395
CCTAATA14600.010.8698462
ACCGTGC4158.1408325E-710.8067218
TAATACT22050.010.7955744
GTATTAA16800.010.67855551
TAAGGGT11650.010.66060454
CATATAC18450.010.6581963
Produced by FastQC (version 0.11.2)