FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359403
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1258.7439534E-519.3195888
TCCGCAC2053.8251528E-818.511864
TAGGTAG3600.018.2079455
GCGCGTC950.007832807518.15884436
CAGTCCG950.0078361318.1575099
TAGACTG2601.7698767E-917.2496345
CCGCACC2402.3100802E-715.81216265
GTATTAG6000.014.9502311
TATACTG9250.014.9186015
CGCACCT3051.4653779E-814.70460466
GAGCCGA1657.2739075E-414.63605111
GCCCCCC1450.005216274414.27555760
CTAGACT1750.001135213213.7997064
TAGACAG4001.9099389E-1013.7997065
GCGCACT1750.001135213213.79970611
CAGGGGT1500.00653056413.79970554
ACAGCGT3801.3005774E-913.6181318
ACTATGC6350.013.5823888
GTCCTAG2304.271011E-513.5002091
CCTAATA7450.013.4292442
Produced by FastQC (version 0.11.2)