FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849957
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT953.201185E-729.05362340
GACGCGC953.201185E-729.05362338
ACGCGCA1057.6773176E-726.28661239
CCGTCGG800.002899233321.56196813
TATACTG5450.020.889395
CTAATAC4700.020.5526833
TGACGCG1356.8178833E-620.44514337
TCTAGAC2406.0754246E-1018.6870373
CGCACCT2105.0336894E-818.07098266
GTGACGC1602.9480205E-517.25058736
CACGAGC1200.001476594217.24957369
TCTATAC3850.017.0255553
TAGACTG2251.10685505E-716.866255
TAGGTAG2057.750132E-716.8288525
TAATACT7100.016.0348134
CGCATGA1958.973939E-615.9236242
GCGCATG1958.973939E-615.9236241
ACTATGC3900.015.9226838
CCCTATG1300.00252140215.9226831
TAGGTCG1300.00252140215.9226835
Produced by FastQC (version 0.11.2)