FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683419
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4850.020.62839966
TAGGTAG6800.016.2349265
TATACGG9850.016.111315
GTATTAG11800.016.0807441
CTAATAC12700.016.0271973
CCGCACC5600.016.01749465
TCCGCAC5500.015.68146364
ACTATGC10500.015.4425088
CATATAC18850.015.1906373
CTAGACA7150.014.4751974
TCTAGAC6200.014.4674143
ACGGTGT9150.014.3275448
TAGTCAG6050.013.685649
TATACCG8600.013.6392265
CTATGCA9950.013.52230559
ACAGCGT7150.013.5101838
CCCCTAG2304.2740823E-513.5001961
TATACAG13900.013.4025745
GTATAAC12000.013.2251941
GTATAGA13050.013.2185831
Produced by FastQC (version 0.11.2)