FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699694
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4900.016.8973164
CTAATAC12200.015.8360433
CACGAGC6400.015.63221369
TAGGTAG6000.015.524985
CCGCACC5800.015.46492365
CGCACCT5650.015.264966
GAGCCCC3857.2759576E-1215.23318169
CTATGCA8750.014.9822829
GCCGTCT7150.014.95774546
GTATTAG10900.014.8758521
TATACGG8450.014.6982055
TATACTG18550.013.9487685
ACTATGC11250.013.7999818
TAGTCAG5500.013.7994719
TGCCGTC8050.013.71401345
GTATGCC7900.013.5387142
TAATACT18300.013.3852294
TTATACT16700.013.22153954
CATCTCG7100.013.1204336
GTATAGA11850.013.10100651
Produced by FastQC (version 0.11.2)