FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519479
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9400.014.3135751
TCGTGCG2454.9645096E-614.0808239
ACCGTGC2651.1158758E-513.0186368
CGCGCGT2701.3531673E-512.77704210
GTATATA21050.012.6197271
GTATAGG7450.012.5031321
ACTATGC9400.012.4785018
TATACTG13200.012.2838725
GTATAGA10700.012.2521191
CTTAGGC2852.3564275E-512.1050473
CATATAC14450.011.9375043
GTATTAT19150.011.8901271
CTAATAC11700.011.7946613
TATATAC25450.011.6579463
GACTAGG17800.011.24092769
ATCGCGC2150.005320959711.2323588
CCTAGCG3451.36008475E-511.00067730
TATACAG14750.010.9930255
CCTAGGA2853.1591285E-410.8945432
GGACCGT3207.681921E-510.7810586
Produced by FastQC (version 0.11.2)